Tag | Content |
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EnhancerAtlas ID | HS180-00406 |
Organism | Homo sapiens |
Tissue/cell | Small_intestine |
Coordinate | chr1:12206530-12213930 |
TF binding sites/motifs | TF | JASPAR ID | Coordinate | Motif Sequence | Strand | -Log10(p-value) |
FOS | MA0476.1 | chr1:12213290-12213301 | TGTGACTCATC | + | 6.14 | HES2 | MA0616.2 | chr1:12208856-12208866 | GGCACGTGCC | + | 6.02 | HES2 | MA0616.2 | chr1:12208856-12208866 | GGCACGTGCC | - | 6.02 | IRF1 | MA0050.2 | chr1:12208704-12208725 | TCTTTCTTTCTTTTTTTTTTT | + | 6.25 | IRF1 | MA0050.2 | chr1:12212158-12212179 | AAAGAAAAAAAGAAAGTGGTG | - | 6.29 | JUND | MA0491.1 | chr1:12213290-12213301 | TGTGACTCATC | + | 6.14 | Klf1 | MA0493.1 | chr1:12208585-12208596 | TGGGTGTGGCT | - | 6.14 | MITF | MA0620.2 | chr1:12209691-12209709 | CATTGTCATGTGACCTCA | + | 6.06 | MITF | MA0620.2 | chr1:12209691-12209709 | CATTGTCATGTGACCTCA | - | 6.06 | PLAG1 | MA0163.1 | chr1:12212500-12212514 | GGAGCCCAAGGGGG | + | 6.39 | RARA(var.2) | MA0730.1 | chr1:12209701-12209718 | TGACCTCAGAGGGACCT | - | 6.08 | SPIC | MA0687.1 | chr1:12213408-12213422 | TTCTTCCTCTTTTG | - | 6.62 | Sox6 | MA0515.1 | chr1:12209832-12209842 | AAAACAATGG | - | 6.02 | ZNF263 | MA0528.1 | chr1:12207125-12207146 | AAAGGAGGTGAGGGGGGAAGG | + | 6.38 | ZNF263 | MA0528.1 | chr1:12211538-12211559 | ACCTCATCTTTCTCCTCCTCC | - | 7.23 | ZNF263 | MA0528.1 | chr1:12211541-12211562 | TCATCTTTCTCCTCCTCCTCC | - | 8.2 |
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| Number of super-enhancer constituents: 56 | ID | Coordinate | Tissue/cell |
SE_00369 | chr1:12202440-12211059 | Adipose_Nuclei | SE_00369 | chr1:12212169-12214461 | Adipose_Nuclei | SE_01471 | chr1:12212830-12213765 | Adrenal_Gland | SE_04546 | chr1:12209905-12211237 | Brain_Anterior_Caudate | SE_06521 | chr1:12204744-12208831 | Brain_Hippocampus_Middle | SE_06521 | chr1:12209695-12211557 | Brain_Hippocampus_Middle | SE_09154 | chr1:12199965-12214486 | CD14 | SE_10696 | chr1:12207898-12214373 | CD19_Primary | SE_11902 | chr1:12207950-12208858 | CD3 | SE_11902 | chr1:12208865-12214231 | CD3 | SE_14553 | chr1:12206658-12208006 | CD4_Memory_Primary_7pool | SE_14553 | chr1:12208809-12214332 | CD4_Memory_Primary_7pool | SE_15480 | chr1:12208768-12214030 | CD4_Memory_Primary_8pool | SE_16369 | chr1:12209491-12212458 | CD4_Naive_Primary_8pool | SE_16369 | chr1:12212506-12213878 | CD4_Naive_Primary_8pool | SE_16946 | chr1:12210087-12214122 | CD4p_CD225int_CD127p_Tmem | SE_17650 | chr1:12208923-12214185 | CD4p_CD25-_CD45RAp_Naive | SE_17827 | chr1:12206501-12214454 | CD4p_CD25-_CD45ROp_Memory | SE_18389 | chr1:12206868-12214379 | CD4p_CD25-_Il17-_PMAstim_Th | SE_19117 | chr1:12206519-12208806 | CD4p_CD25-_Il17p_PMAstim_Th17 | SE_19117 | chr1:12208827-12214340 | CD4p_CD25-_Il17p_PMAstim_Th17 | SE_20048 | chr1:12207907-12214477 | CD56 | SE_20873 | chr1:12208284-12214108 | CD8_Memory_7pool | SE_22347 | chr1:12207903-12214404 | CD8_primiary | SE_26251 | chr1:12206411-12213727 | Duodenum_Smooth_Muscle | SE_27503 | chr1:12206710-12207876 | Esophagus | SE_27503 | chr1:12207969-12208776 | Esophagus | SE_27503 | chr1:12209763-12212109 | Esophagus | SE_27503 | chr1:12212703-12214088 | Esophagus | SE_30788 | chr1:12206605-12208826 | Fetal_Muscle | SE_30788 | chr1:12209786-12211445 | Fetal_Muscle | SE_30788 | chr1:12212889-12214071 | Fetal_Muscle | SE_32030 | chr1:12206339-12207888 | Gastric | SE_32030 | chr1:12207925-12209356 | Gastric | SE_32030 | chr1:12209823-12210827 | Gastric | SE_32030 | chr1:12210903-12211697 | Gastric | SE_32030 | chr1:12212751-12214118 | Gastric | SE_38661 | chr1:12206185-12208882 | HUVEC | SE_38661 | chr1:12212782-12214329 | HUVEC | SE_41498 | chr1:12206022-12208803 | Left_Ventricle | SE_41498 | chr1:12209754-12211519 | Left_Ventricle | SE_41498 | chr1:12212621-12214203 | Left_Ventricle | SE_42336 | chr1:12205947-12208671 | Lung | SE_42336 | chr1:12208734-12209640 | Lung | SE_42336 | chr1:12209781-12214078 | Lung | SE_48967 | chr1:12206113-12207889 | Right_Atrium | SE_48967 | chr1:12207966-12208776 | Right_Atrium | SE_48967 | chr1:12209749-12211554 | Right_Atrium | SE_48967 | chr1:12212801-12214137 | Right_Atrium | SE_50250 | chr1:12206451-12207889 | Sigmoid_Colon | SE_50250 | chr1:12207958-12214180 | Sigmoid_Colon | SE_52522 | chr1:12206320-12214007 | Small_Intestine | SE_53313 | chr1:12206186-12214442 | Spleen | SE_55394 | chr1:12209790-12211650 | Thymus | SE_59216 | chr1:12197780-12224862 | Ly3 | SE_61096 | chr1:12184746-12245090 | HBL1 |
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| Diseases: AD,Huntington,Obesity,Parkinson,Prostate cancer,Schizophrenia and Sleep disorder | Number of disease enhancers: 2 | Chromosome | Start | End |
chr1 | 12210430 | 12211346 | chr1 | 12213128 | 12213883 |
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| Number: 1 | ID | Chromosome | Start | End |
GH01I012140 | chr1 | 12200161 | 12214656 |
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Enhancer Sequence | ACCTAGCCAC CCTTGGGGCC AACTTGCTGT GGGAAGCCCC GCAGTGGGCA GGCCTCTTCT 60 TGTTCTGTGG GCTACCCCAA GAACCCTTCA GATCACAGTC ATAGGACTGG CTCATTATTC 120 GACGTCTGTA ACTCTGGGCA TGTCGGCGAA GTGCTGCGGG TCCCTCCGGA GGGACATCTC 180 ACTCGTGGTT CTAGCACATT CCAGATTGCA CCACATTACT GTTCGAGGGC TTTCCTCAAT 240 TAAAGGGTCT AGCTGCTGAG GACTAGTGGG GCACAGACTT GCTCTCGTGG GTCCTGTGTC 300 TGGTGGACGG GACAGACGGT GGCGTCATCA AATCAGTACA CAAGGTCATT CCAGAGTCAG 360 ATCACGCTGT GAACCACAAG GAGTCTGAGC CAGGTACTGT GGCAGAGTGG GAGGGGAGGG 420 CCACCACTAG CTAGGTGGTC AGGGCAAGCC TCTTTGAGAA GGTGCCCTTT GAACTTGGCC 480 TGAGTGATGT CACAAGCCAG CGAGGTCTGG AAGGCAGGGA GCTGAAGTGG GCACACTCCT 540 GCCTCGAGTT TCAGGAAGAA CACAGTGGTG GAAGAGGCAC GGTGGGCGAG GGAAAAAAGG 600 AGGTGAGGGG GGAAGGCCTG GGGAGGAGGG CCTGGTGGGC CATTGTGATT AGGCACCTAG 660 ATTTTATCAT AAAAGTACTG GTCTATGTTT CTTTCTGTTT TCTTCCTAGT AGAACAATAA 720 ACATCCTGGT ATTATTTCAG CAATCAGTGA GAAAGGCCAA ACCAGGGGCG GCAAAGGTTG 780 TTTTAACCTA TTTTGGAGGG AAGTTGGGGA AACAGGCAGC AGCTGCTGGC AGCCAGGAAG 840 CAGTTAGATG GGTTTTCTCA GAAAGAACTA AGACAGCATT CCTGGTGATG ATTGTAGCTA 900 TGGGTAGGGT TAGGAGTCTG CCGGTCTAGC TTCCCCCTTT CTCTGGGTAT CTTCCAGAGA 960 GTAAAAACCA AACTGAAAAA CCCTGGGAAG CCTGGAGGCC TTTGTTTGTG TTTTGGTGTG 1020 GTGATTGTTT TCCCGGTCAT GAGGCAGAGG GATCATTTCA GTATCACAGG GTCAGCGGTG 1080 GCCTGTGCTT TTTACCCTTT AGCCCTCATG GAGGCAGTGG CCATTTCATG ACGGTGGCAT 1140 GGAGCTGTGG CTTCCGGCTC GACAAGCTCC CAGCTGCTTG GTCCCAAGCA AAGCCCTTAT 1200 GTGCGTTCAA TGTGGGTTTT CTGGTCTGCA AAATGGACCT AATGGGGCCA GATGCGGTGG 1260 CTCACGCCTG CAATCCCAGC ACTTTGGGAG GCCGAGGTGA GTGGGTCACT TGAGGTCAGG 1320 AGTTCGAGAC CAGCCCGGGC AACATGGCAA AACCCCGTCT CGACTAAAAA TACAAAAAGT 1380 AGCCAGGCAT GGTGGCATAT GCCTGTGATC CCAGCTACTT GGGAGGCTAA GGTGGGAGAA 1440 TTGCTTGAAC CTGGGAGGCG GAGGTTGCAG TGAGCCGAGA TCGTGCCACT GCACTCCAGC 1500 CTGGGTGACA GAACGAGACT CTGTCTTCAA AAAAAAAAAG AAAAGGAACT ATAAGGCTGA 1560 GAGGGACTGT GGAAGTCCAG TCTGGGCACG TATGCCATGT TCAGTAAATG ACAGCCATCA 1620 AGAGCAGCAT CTTAAGAGCT GAGAGTTCGC CTAGGCCGCA TTCTTTGTAC ATCTGGGCTT 1680 CCTAATAGAA CCCTGGACAG TAGATAGGCT GGGTGGCAGC ACTCCCTTTT ACAAACTGAG 1740 GCTCGGGGAG TGGGGGGGAG CGAAGCAACT GGCCCACTGC CACACAGCTG GGAAGTCCCA 1800 GAGCTGGAGT TCAACCCCCA ACTTTGTGAT TCTAAATCCC TCCCCATGCC CAGGTGGCCT 1860 GGCTGCCTCT GTGGTCAGGG GTTGTGTTCA TATCGTGGTC ATCCCCTGGC ACTATGCCTT 1920 CTAGATAAGT TTCCTTTCAT CTTCAAGTCA CCACAACTGA GAGATAGAAT AGAGGCTGAT 1980 TTTATTCCTC CCTTTATGGG AAGGCAGGGA CAGTTGCAAG AGGCAGGGTA ACCTGCCCAA 2040 GGTCAAACTC CAGGCTGGGT GTGGCTTTCT GACTGCTCTG ACCGGACAGG AAGGACACAG 2100 AGCTAACCCA GCCAGGGCCT CCTTCCTTGA CACAGCTTTC CTCAAAGGTG TGTGTGATTT 2160 TTTTTTTCTT TCTTTCTTTC TTTCTTTTTT TTTTTTTTTT TTGAGACAGG GTCTTCCTCT 2220 GTCACCCAGG CTGGAATGCA GTGGCACAAA TACAGCTCAC TGTAGCCTCA ACTTCCTGGG 2280 CTCACGTGAT CCTCCTGCTT CAGCCTCCCG AGTAGCTGGG ATCACAGGCA CGTGCCACCA 2340 TGACTGGCTA ATTTTTTTTT TTCTTTCTTT GTAGAGACAG GTCTCTCTAT GTTGCCCAGG 2400 CTGGTCTTGA ATTCCTGGGC TCAAGCAATC TTCCTACCTT GGCTTCCCAA AGTACTGGAT 2460 TACAGGCGTG TGATTCTGAG GTCCAGGGAG ACTCGGGGCT GGGTTGAGTC CAGGGAATCT 2520 CTGGGGGAGA ACTGCCTGAG AGTGGAATTG CCCTTTCCAT CCCCAGTAAC ACCTCATATC 2580 CTGCTCACTG CCCCACCTGA GGCTCCCTGA AGGAAGACGA TCAAGAACAA GCCTCCCATT 2640 TTACAGATGG GAAAACTGAG CCCCATAGAG GCAAAGCAGT TTTCCCAGGG TCCCAGCTTG 2700 GTGCTGTGTG ATGGAGATAG CTCTAACTGT AGCTGTCAGT TGGGGCTGGG GTAGGGGAAA 2760 GTCACTGTCT AGTCCAAGGG AGCAGTAAAG ATGACATGCT GATTTTCATT CTTCCTTTAA 2820 AGAAAAAATT GATGTTAAAA GTAACTGTGG TGAGAGGGTT GGGTTCTTAC TTACAAGGCA 2880 GCTTTGCAAG AGTAAACAGA GTAGACAAAA ATATCCCATC AGCTGGGCTT CTGCAGCTTC 2940 ACAAGCTACT GATCCGTGCA GTCAGAGGGC CCCAGGTTCA GAAGGGCCCT GGGCTTGGTT 3000 TAATGCTCTG CTGTCGCCAT CTTGAAAGTG ATGAATAATT TTTGGCAAGG GGCCCTGTGT 3060 TTTCATTTTT CATGGAGTTC TACAAATTAT GTAGCTGATC TTGCATGGGG GCAGGGAAAT 3120 CGTTAATTCA GAATTCACTT AATGCCAAAG CATTTTACCT CCATTGTCAT GTGACCTCAG 3180 AGGGACCTTA TAGATGAGCA CACATGTCCC CATTTTTAAG AAGTGTTAAA TGACCAGTAA 3240 CTGATGTAAC TGTTCCAGTC ATCTCTTGCT ACATAACAAA TCACCTCAAA ACTTAATAGC 3300 TTAAAACAAT GGTCATTAAT TTTGCTCACA AATCTGCAAT GTGGCCTGGA CTTAGCAGGG 3360 GAGGTGCATC TCGGCTCACA TAGTACCAGC GGGGGTGATG AGATGAGCTG GAGAATCCAC 3420 CTCCAAGAGG GCTGGCAAAT TGGTACTGGC TGTCAGTTTC TCTCTGTGGA GCTTCTTGGG 3480 CTTCCTCACA GCATGGCTGC TGGGTTCCTG GAGCCAACAA CTCCGGGGGC AGGAAGTAGA 3540 AACTGCCAGC GTCATAAAAT TTGACCCCGG AAACTGGCAC TGCATCCCCT TTGCTGCATT 3600 CCAGGTAGTC ACAGAGCCCA GACTCAAGGG GAGGGGACAC AGATCCCACC CCTCATTAGG 3660 AGGAGTGTCA AAGAATCTCG AGATCTTATG TGTCAGCAGG AGCACTTCAC ATACTGTCTC 3720 ATTTTAGCCT CTCGGTAATT TTGCCATTAT TGTCCTTGGA GTTGGCTCAA GGAAGCTACA 3780 TAACCTGGTG GAATCCCAAG TTCCTCTGGC TGGAATTTGG ATCCAAGCTG GTCTGTCTCC 3840 AGAGTGTGAG CTCTGGACTG AGCAGCTGAC CTGTGCATTC AGGGACCCAT ACAGTCAGAA 3900 TGTGGCAGGA CTGGAAAGCA AGCTCACATC TGTCTGATTA GAGAACATCT GAGCTTGGTA 3960 CCCATACAAA CTAGGCCCTA TCTAGTTTTT AGATAGGGAA ATCAAGGCTC TGAGAGGTCT 4020 TGCCAGGCCT CTCCTGAGGA AGAGGGCAGG GTCTGGGACC TTCCAGGGCA GGATGGGGCC 4080 CCCAACTTGG GTTGCACAGA ACCATGTCAG GAAGTCACAT AGAGTTGTGG GTAGATTATG 4140 CAAGTACAGG CTGTCAGGAG GGATGTTGTT TTTCACTGCA ATTACACTTC ACTCAGGAAA 4200 TACAGCTTCT GCACGTACTG AGGGAGGGTT CCTCACCCTG TGCAGAAGAC CTCCTGGTGA 4260 ATACCGGGGC ACTCGCTGGG TGACCCTGGG TCCTCTGGGC CAAGTTCATG CATCGAAAGT 4320 TCCCTGAGAA GCTCTTTGTG CCCATCTGGG TTGGGCTTTG ACAATAGAGT TGAAGAGGCT 4380 TTATTCCTGC CATGCATGGA GTCACAGGTT AGTAAGAAAG ATGATAAGAG CTGTACTTGA 4440 GGCTTTCGTG TGCTCTCACT TAAACCTCAC AGTGCACTAG GAAGTAGTTA TTACATTACC 4500 CCTATGTTAC AGATGAGGAA ACCAACACGC AGAGTGGCTA AGCCAGTTCC CCAGGGTCAC 4560 ACAGCTGCTA AGTGGCGCAG CTAGGCTTTG AACCCAGGCC ACCTGGCTGG AGTCTGCGTC 4620 CTCACACACT TGGCCATGCT GCTCCATTGT GGCTCAGGCC GAGGTCACCA CTGTGCTGAG 4680 GCCCCAGCCC TGAGTCCCAG ACCCTGTAGA ACACTGTGCA GGCATCCATG GGGCTCAGTG 4740 AATGCACAGG GAATGGAATT TGCTCTTGGT TTTTATCCCA CCTTTCCTTT CCAAAGCCCA 4800 GCTGTGGCCA GGCCTCTCCC TCACAGAACC TTCTATGACT CCCTAGTGCC TACATGCCAA 4860 TGTCTGCCAA TCCAGCTTTG CTGCTACCTC CACAGCTCCC ATATCTAAAC CTCTCCTGAG 4920 ACCCGACTGT TTCCGCTCAG TCACCAAATA CAGAGGGCAT ATCCTCATCA TGAAATCTCT 4980 GCTCAGCGCT CCAACCCCTG AATTTCCAAC CTCATCTTTC TCCTCCTCCT CCAAAAACCC 5040 TTCCAGGACA CCCAGGCCCA GGACCTCCAA GCTCCCACAG CACTGACTGT GAAGGGGGAC 5100 TGGAGGCCAA GTTCTAGAAG TCTTGCAAAA AATATCTCTG AAGAAATGCA TTCATTCATT 5160 CATACAGCCA CTCATTTAGT CACTCAAGCA ACAGATCTGT ATGTAACACC TACTATGTGT 5220 TGGGCACTGT GCTTGGCGCT GGGGAACACA GTGAAGTGGG GCAGTGGCGG GGATATACAG 5280 TACTGCTCTC AGGGAGTCAC AGTCCAACTG GGAGACCAAC CTAGAGTCTG GTCATGGCCT 5340 AGAGCATGAT ATTTGCTTTG ATGGAGAGCA GCTTGGGGTT CATGACCCTA GTGGATGGCA 5400 CCTAGCCCAG ATGGAGGCAG GTCTCTGCTT TCCCCTCTGT AGAATGGGTT TAATAAGAGC 5460 ACCTACCCCA AAGAGATGTT GTAAGGATTA ATTTCATCCA TTAATTTGTT CAATAAATAT 5520 TTGTCAAGCT CAAACTCCGT GCTGGGGTGC TGGGGACACA CAGCCCTGCC CTCTGCAGAG 5580 TGACAAGAAA GAGTATTTGG GATAACATTG GCTGGTGGTA ATTGCTGTAA AGAAAAAAAG 5640 AAAGTGGTGT GTAGGCCCTC AGTGTGGTGA GGTGTGTAGC CAGGGCTTGG GGATGTAGCA 5700 CCTGCTGTAC AAAGGTCTGG GAGAATGTAG CGATTCAGGG TGTGCAGGGG GAAGTGAGTA 5760 GTTATGATCA TGAGTCTGTG GTAGACATCG CCCCATCCTT GCATTTGCAT CTCTAGGTTT 5820 CTACAGAAGA AAGGGGGCAA GGTGATGGAG AGAATTTCAT GGGCGGAAAT TGTTCTACAG 5880 GAGCTGGGGA GGTAGGCTGG CAGGGCTGAG CCGGCTACTT GGTGGGAGGT TGCAACAGCC 5940 TCAGGACGGG TGGAGGACTG CAGGGGAACT GGAGCCCAAG GGGGTTTCCT TCCAGGGTGA 6000 AGGGGAAAAG CAAGGCTGGG CACTTTGGGA ACTTCTCAGG GAAACCCTCT CCTGTACCCT 6060 AGGGGACCTG GGCTCCATTC TGGAAAGGCA GAATACATGA CCTCCATCAC GTTTTGGGTC 6120 TGCACTCATT AAGCACTTAC TAGATGCCAG GCACTGTAGA GTCTCCATCT CAGATCCTTA 6180 CAGCACCCCT GTAAAGCAGA GGCTATTTAT CCCCATTCTA CAGATGAAGA AATGGAGGCT 6240 CAGGGAGGTA AAGTGATTTG TCCAAGGTCA TGGTTGCGTA CCAGTCAATG GCCTGAACAG 6300 AGACTTGAAC CCAGGTCTAG GTGATGGCAA AGTTGATGCT ATTTCTCCTT TTGATATACC 6360 CCAACTGTCT AAAGCAGCCC TTTTTTTTGC TAGGCTTCTT GGTGTTTGAA AATTTACCAC 6420 TCATTCCTAG GAGCTAAAGG TTCCTGGGTT CCTCCTTGGG GCACATGTCT AGGAGCAGGA 6480 TCTCGATCTG GTGGCAGAGG GGCAGCAGCT GCTCCGTCTG GCATACAGTA GGTGCTCAGT 6540 AAGTCTGCGG GGCAGGCAGG ACAAGCCTGG GTTGGAAGGA GGTCCAGTTT CTTGGGAGTG 6600 AGAGCCTCAA GACCAACTTC ACCTGAAGGA CAAATGAGAG GTGGCCGAAT CACTGCCAGC 6660 TACTTCAAGA AGCCCCTAGG ACACTCCAGC CAAGGTGCCT TGGTCCCTTT TCTAAGTCGC 6720 CTGCCCACCC TGCCCAAGCT CCTGCGGGGA GTGTGCCCAC TGTGACTCAT CCACGCCCAG 6780 CCCTGCCCAG CTCTCACTGG GGCAGCAGTA GGCACACATA CTCGTAAAAG TCTGAATAAG 6840 TGAAGACTGG GAAATTGCAC CAGGGAAAAA CTACTTATTT CTTCCTCTTT TGAATTGGGG 6900 AGGGGAAATG TTGCGGGGGA AATGAGGTCA GTGACGTGGT GAGCATTTCA TGCTCCTTAC 6960 TGATGAGCAG AGGGCCGGTA TCACGTGCTG CCTTCCTAGG GAGGGCTGGG TAGTGCGTCA 7020 AGGAAATGGG CCCAGAGCTG TGTCTCGTCC AGTCTAGCAG GAAACTGCCC ATCTCACTGT 7080 GTCACTCCCT TCTCTGTCCT TGCTCTAAGC CTCTACCCTG ACTGCCCTTG CTCTGAGCTG 7140 AAATCTCACT AATGAGAAAA AAATGTGCTG GTTTATGCCT CTGTGGGCTG GCTGGGCTGG 7200 TAGGTCCCAG GGGGCAGCAT GGCCTGGAGG AAACCGAGTG CACGGGGCAA GGATGATGTG 7260 CACGAGTGGT ATGTGACTCC CAGGGCTCAC AGCCTCATGC AGCCCCTCCC TCTGAGAGTA 7320 TAGATGGGGA AACTGAGGCC TGAGAAGGCG AGAGATGTGC CTGAGGTCAC ACAGAGAATC 7380 CGTGGCCCTC AGCACAAGCA 7400
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